polyclonal rabbit anti human ccr7  (Bioss)

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Gene Expression Analysis in the Overall Study Cohort

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Gene Expression Analysis with Respect to CRS Clinical Phenotype Compared to Controls

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Spearman Correlations Between Genes in the Overall Study Cohort

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Spearman Correlations Between Genes and CRS-Specific Disease Severity Metrics in the Overall Study Cohort

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Sinonasal epithelial cell expression of CCL19 and its receptors CCR7 and CCRL1 is elevated in CRSwNP (n=7) compared to in CRSsNP (n=6) and controls (n=6). ( A ) Representative immunohistochemical images demonstrate the increased co-expression of CCL19 and typical receptor CCR7 (top), as well as of CCL19 and its atypical receptor CCRL1, in the sinonasal epithelium in patients with CRSwNP compared to in CRSsNP and controls. CCL19 appears gray, whereas its receptors appear dark brown. ( B ) CCL19 and CCR7 co-expression in the sinonasal epithelium, represented as the % total area via Image J analysis, is significantly elevated in CRSwNP compared to CRSsNP ( p =0.04) and controls ( p =0.06). ( C ) Although not significant, CCL19 and CCRL1 co-expression is elevated in CRSwNP compared to in CRSsNP and controls, represented as the % total area via Image J analysis. Data is represented by individual values with the mean ± SD with respect to cohort.

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing, Immunohistochemical staining

Journal: Journal of Inflammation Research

Article Title: Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

doi: 10.2147/JIR.S453567

Figure Lengend Snippet: Summary of Key Results

Article Snippet: The tissues were then incubated with polyclonal rabbit anti-human CCR7 (1:200, 4°C, overnight) (Bioss Antibodies, bs-1305R; Woburn, MA, USA), followed by incubation with ImmPRESS TM HRP anti-rabbit IgG (20 min) and detection with ImmPACT DAB Peroxidase kits from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing

Journal: Journal of Virology

Article Title: Prostaglandin D2 delays CD8+ T-cell responses and respiratory syncytial virus clearance in geriatric cotton rats

doi: 10.1128/jvi.01863-24

Figure Lengend Snippet: Lung DC activation and migration to lymph nodes are impaired in geriatric cotton rats. Tissues were collected at the indicated time points post-RSV infection, with 0 hours post-infection representing uninfected cotton rats. ( A ) A significantly lower (* P < 0.01) percentage of lung DCs (CD11c+/MHC-II+/Siglec F-) express CCR7, a marker of DC activation and migration to lymphoid tissues, at 24 and 48 hours post-infection in geriatric cotton rats compared with adults. ( B ) The proportions of DCs within MLN of adult cotton rats increase to significantly higher percentages (* P < 0.01) at 48 and 72 hours post-infection compared with geriatric animals, suggesting decreased DC migration from the lung in geriatric cotton rats. ( n = 4 for adult 0 hours p.i. in A. n = 5 for all other data points.)

Article Snippet: Proportion of lung DCs expressing CCR7 (cross-reactive polyclonal rabbit IgG anti-human/mouse/rat CCR7, immunogen amino acids 25–59 of mouse CCR7, Thermo Fisher) were measured within the MHC II+/CD11c+/Siglec F- population.

Techniques: Activation Assay, Migration, Infection, Marker

Journal: Journal of Virology

Article Title: Prostaglandin D2 delays CD8+ T-cell responses and respiratory syncytial virus clearance in geriatric cotton rats

doi: 10.1128/jvi.01863-24

Figure Lengend Snippet: COX-2 inhibition increases pulmonary DC CCR7 expression and RSV-specific CD8+ T cell responses. RSV-infected geriatric cotton rats were treated daily with the COX-2 inhibitor NS-398 intraperitoneally from days 0–1 ( A ) or 0–4 ( B ). (A) At day 2 post-infection, a cohort was euthanized, and the lungs were collected to measure CCR7 expression in DCs (CD11c+/MHC-II+/Siglec F-) by flow cytometry. Compared with untreated geriatric cotton rats, NS-398 treatment resulted in higher CCR7-expressing lung DCs (* P < 0.01). (B) At day 8 post-infection, lung and MLN were collected to measure RSV-specific CD8 T-cell responses. The percentage of RSV-specific CD8+ T cells was calculated as the increase in the percentage of IFN-γ expressing CD8+ T cells in peptide-stimulated samples from unstimulated matching controls. COX-2 inhibition resulted in an increased RSV-specific CD8+ T-cell response in MLN (* P < 0.01) but not the lung. ( n = 5 for all data points).

Article Snippet: Proportion of lung DCs expressing CCR7 (cross-reactive polyclonal rabbit IgG anti-human/mouse/rat CCR7, immunogen amino acids 25–59 of mouse CCR7, Thermo Fisher) were measured within the MHC II+/CD11c+/Siglec F- population.

Techniques: Inhibition, Expressing, Infection, Flow Cytometry